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Erythropoiesis

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Cover of 'Erythropoiesis'

Table of Contents

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    Book Overview
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    Chapter 1 An Introduction to Erythropoiesis Approaches
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    Chapter 2 Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis
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    Chapter 3 Mouse Models of Erythropoiesis and Associated Diseases
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    Chapter 4 Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells
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    Chapter 5 Stress Erythropoiesis Model Systems
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    Chapter 6 Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models
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    Chapter 7 Functional Analysis of Erythroid Progenitors by Colony-Forming Assays
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    Chapter 8 Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands
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    Chapter 9 Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells
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    Chapter 10 Analysis of Erythropoiesis Using Imaging Flow Cytometry
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    Chapter 11 Flow Cytometric Analysis of Erythroblast Enucleation
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    Chapter 12 High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
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    Chapter 13 Chromatin Immunoprecipitation (ChIP) with Erythroid Samples
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    Chapter 14 Chromosome Conformation Capture (3C and Higher) with Erythroid Samples
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    Chapter 15 Genome Editing of Erythroid Cell Culture Model Systems
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    Chapter 16 In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood
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    Chapter 17 Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines
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    Chapter 18 Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products
Attention for Chapter 6: Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models
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Chapter title
Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models
Chapter number 6
Book title
Erythropoiesis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7428-3_6
Pubmed ID
29076086
Book ISBNs
978-1-4939-7427-6, 978-1-4939-7428-3
Authors

Marie Trudel, Josepha-Clara Sedzro

Abstract

Over the last decades several mouse models of human hemoglobin disorders have been and continue to be generated. This chapter aims at describing various approaches to evaluate the global red blood cell properties in mouse models of human hemoglobin disorders, in particular thalassemia and sickle cell disease. Analysis of erythroid parameters provides insights into the RBC physiologic or pathophysiologic status. Mice expressing both murine and human globin genes can be investigated using adapted protocols provided herein.

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 50%
Student > Ph. D. Student 1 25%
Professor 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 75%
Medicine and Dentistry 1 25%

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