| Chapter title |
Chromosome Conformation Capture (3C and Higher) with Erythroid Samples
|
|---|---|
| Chapter number | 14 |
| Book title |
Erythropoiesis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7428-3_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7427-6, 978-1-4939-7428-3
|
| Authors |
Ivan Krivega, Ann Dean |
| Abstract |
Chromosome conformation capture (3C) allows for the determination of the proximity in nuclei of DNA sequences that are linearly distant from one another in the genome. Proximity that is above that expected from random interaction provides evidence for potential long-range functional interactions such as between enhancers and their target genes. Many controls are required to convincingly demonstrate increased frequency of interaction between sequences and stringent functional tests must also be applied. Here, we present methodology suitable for 3C experiments that can also be applied as the basis for related 4C, 5C, and Hi-C approaches. These procedures are widely applicable to erythroid cell lines, progenitor cells, and tissues. |
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|---|---|---|
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Demographic breakdown
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|---|---|---|
| Researcher | 4 | 50% |
| Student > Ph. D. Student | 1 | 13% |
| Student > Postgraduate | 1 | 13% |
| Unknown | 2 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Unknown | 2 | 25% |