↓ Skip to main content


Overview of attention for book
Cover of 'Erythropoiesis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 An Introduction to Erythropoiesis Approaches
  3. Altmetric Badge
    Chapter 2 Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis
  4. Altmetric Badge
    Chapter 3 Mouse Models of Erythropoiesis and Associated Diseases
  5. Altmetric Badge
    Chapter 4 Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells
  6. Altmetric Badge
    Chapter 5 Stress Erythropoiesis Model Systems
  7. Altmetric Badge
    Chapter 6 Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models
  8. Altmetric Badge
    Chapter 7 Functional Analysis of Erythroid Progenitors by Colony-Forming Assays
  9. Altmetric Badge
    Chapter 8 Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands
  10. Altmetric Badge
    Chapter 9 Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells
  11. Altmetric Badge
    Chapter 10 Analysis of Erythropoiesis Using Imaging Flow Cytometry
  12. Altmetric Badge
    Chapter 11 Flow Cytometric Analysis of Erythroblast Enucleation
  13. Altmetric Badge
    Chapter 12 High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
  14. Altmetric Badge
    Chapter 13 Chromatin Immunoprecipitation (ChIP) with Erythroid Samples
  15. Altmetric Badge
    Chapter 14 Chromosome Conformation Capture (3C and Higher) with Erythroid Samples
  16. Altmetric Badge
    Chapter 15 Genome Editing of Erythroid Cell Culture Model Systems
  17. Altmetric Badge
    Chapter 16 In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood
  18. Altmetric Badge
    Chapter 17 Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines
  19. Altmetric Badge
    Chapter 18 Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products
Attention for Chapter 12: High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
Altmetric Badge

Mentioned by

1 tweeter

Readers on

3 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
Chapter number 12
Book title
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7428-3_12
Pubmed ID
Book ISBNs
978-1-4939-7427-6, 978-1-4939-7428-3

Alyson S. Smith, Roberta B. Nowak, Velia M. Fowler, Smith, Alyson S., Nowak, Roberta B., Fowler, Velia M.


During erythropoiesis, erythroblasts undergo dramatic morphological changes to produce mature erythrocytes. Many unanswered questions regarding the molecular mechanisms behind these changes can be addressed with high-resolution fluorescence imaging. Immunofluoresence staining enables localization of specific molecules, organelles, and membrane components in intact cells at different phases of erythropoiesis. Confocal laser scanning microscopy can provide high-resolution, three-dimensional images of stained structures, which can be used to dissect the molecular mechanisms driving erythropoiesis. The sample preparation, staining procedure, imaging parameters, and image analysis methods used directly affect the quality of the confocal images and the amount and accuracy of information that they can provide. Here, we describe methods to dissect erythropoietic tissues from mice, to perform immunofluorescence staining and confocal imaging of various molecules, organelles and structures of interest in erythroblasts, and to present and quantitatively analyze the data obtained in these fluorescence images.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 67%
Researcher 1 33%
Readers by discipline Count As %
Medicine and Dentistry 2 67%
Biochemistry, Genetics and Molecular Biology 1 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2018.
All research outputs
of 13,116,247 outputs
Outputs from Methods in molecular biology
of 8,452 outputs
Outputs of similar age
of 310,855 outputs
Outputs of similar age from Methods in molecular biology
of 1 outputs
Altmetric has tracked 13,116,247 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,452 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 310,855 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them