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Erythropoiesis

Overview of attention for book
Cover of 'Erythropoiesis'

Table of Contents

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    Book Overview
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    Chapter 1 An Introduction to Erythropoiesis Approaches
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    Chapter 2 Using the Zebrafish as an Approach to Examine the Mechanisms of Vertebrate Erythropoiesis
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    Chapter 3 Mouse Models of Erythropoiesis and Associated Diseases
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    Chapter 4 Dissecting Regulatory Mechanisms Using Mouse Fetal Liver-Derived Erythroid Cells
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    Chapter 5 Stress Erythropoiesis Model Systems
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    Chapter 6 Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models
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    Chapter 7 Functional Analysis of Erythroid Progenitors by Colony-Forming Assays
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    Chapter 8 Analyzing the Formation, Morphology, and Integrity of Erythroblastic Islands
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    Chapter 9 Flow Cytometry (FCM) Analysis and Fluorescence-Activated Cell Sorting (FACS) of Erythroid Cells
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    Chapter 10 Analysis of Erythropoiesis Using Imaging Flow Cytometry
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    Chapter 11 Flow Cytometric Analysis of Erythroblast Enucleation
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    Chapter 12 High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
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    Chapter 13 Chromatin Immunoprecipitation (ChIP) with Erythroid Samples
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    Chapter 14 Chromosome Conformation Capture (3C and Higher) with Erythroid Samples
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    Chapter 15 Genome Editing of Erythroid Cell Culture Model Systems
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    Chapter 16 In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood
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    Chapter 17 Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines
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    Chapter 18 Good Manufacturing Practice (GMP) Translation of Advanced Cellular Therapeutics: Lessons for the Manufacture of Erythrocytes as Medicinal Products
Attention for Chapter 12: High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
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Chapter title
High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure
Chapter number 12
Book title
Erythropoiesis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7428-3_12
Pubmed ID
Book ISBNs
978-1-4939-7427-6, 978-1-4939-7428-3
Authors

Alyson S. Smith, Roberta B. Nowak, Velia M. Fowler, Smith, Alyson S., Nowak, Roberta B., Fowler, Velia M.

Abstract

During erythropoiesis, erythroblasts undergo dramatic morphological changes to produce mature erythrocytes. Many unanswered questions regarding the molecular mechanisms behind these changes can be addressed with high-resolution fluorescence imaging. Immunofluoresence staining enables localization of specific molecules, organelles, and membrane components in intact cells at different phases of erythropoiesis. Confocal laser scanning microscopy can provide high-resolution, three-dimensional images of stained structures, which can be used to dissect the molecular mechanisms driving erythropoiesis. The sample preparation, staining procedure, imaging parameters, and image analysis methods used directly affect the quality of the confocal images and the amount and accuracy of information that they can provide. Here, we describe methods to dissect erythropoietic tissues from mice, to perform immunofluorescence staining and confocal imaging of various molecules, organelles and structures of interest in erythroblasts, and to present and quantitatively analyze the data obtained in these fluorescence images.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 67%
Researcher 1 33%
Readers by discipline Count As %
Medicine and Dentistry 2 67%
Biochemistry, Genetics and Molecular Biology 1 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2018.
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#11,650,868
of 13,116,247 outputs
Outputs from Methods in molecular biology
#6,007
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#264,035
of 310,855 outputs
Outputs of similar age from Methods in molecular biology
#1
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