↓ Skip to main content
What is this page? Embed badge

Phage Display

Overview of attention for book
Cover of 'Phage Display'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Construction of Human Immune and Naive scFv Libraries
  3. Altmetric Badge
    Chapter 2 Construction of Naive and Immune Human Fab Phage-Display Library
  4. Altmetric Badge
    Chapter 3 Construction of Synthetic Antibody Phage-Display Libraries
  5. Altmetric Badge
    Chapter 4 Modular Construction of Large Non-Immune Human Antibody Phage-Display Libraries from Variable Heavy and Light Chain Gene Cassettes
  6. Altmetric Badge
    Chapter 5 Construction of Macaque Immune-Libraries
  7. Altmetric Badge
    Chapter 6 Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format
  8. Altmetric Badge
    Chapter 7 Construction of Rabbit Immune Antibody Libraries
  9. Altmetric Badge
    Chapter 8 Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries
  10. Altmetric Badge
    Chapter 9 Construction of High-Quality Camel Immune Antibody Libraries
  11. Altmetric Badge
    Chapter 10 Construction of Chicken Antibody Libraries
  12. Altmetric Badge
    Chapter 11 Construction and Selection of Affilin® Phage Display Libraries
  13. Altmetric Badge
    Chapter 12 Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies
  14. Altmetric Badge
    Chapter 13 Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach
  15. Altmetric Badge
    Chapter 14 Parallelized Antibody Selection in Microtiter Plates
  16. Altmetric Badge
    Chapter 15 Mass Spectrometry Immuno Assay (MSIA™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning
  17. Altmetric Badge
    Chapter 16 Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection
  18. Altmetric Badge
    Chapter 17 Phage Display and Selections on Cells
  19. Altmetric Badge
    Chapter 18 Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
  20. Altmetric Badge
    Chapter 19 Antibody Isolation From a Human Synthetic Combinatorial and Other Libraries of Single-Chain Antibodies
  21. Altmetric Badge
    Chapter 20 Screening Phage-Display Antibody Libraries Using Protein Arrays
  22. Altmetric Badge
    Chapter 21 Antibody Selection on FFPE Tissue Slides
  23. Altmetric Badge
    Chapter 22 Antibody Affinity and Stability Maturation by Error-Prone PCR
  24. Altmetric Badge
    Chapter 23 Upgrading Affinity Screening Experiments by Analysis of Next-Generation Sequencing Data
  25. Altmetric Badge
    Chapter 24 Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Discovery
  26. Altmetric Badge
    Chapter 25 High-Throughput IgG Reformatting and Expression
  27. Altmetric Badge
    Chapter 26 Monitoring Phage Biopanning by Next-Generation Sequencing
  28. Altmetric Badge
    Chapter 27 ORFeome Phage Display.
  29. Altmetric Badge
    Chapter 28 Epitope Mapping by Phage Display
  30. Altmetric Badge
    Chapter 29 Metasecretome Phage Display
  31. Altmetric Badge
    Chapter 30 Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages
Attention for Chapter 27: ORFeome Phage Display.
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
2 X users

Citations

dimensions_citation
13 Dimensions

Readers on

mendeley
30 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
ORFeome Phage Display.
Chapter number 27
Book title
Phage Display
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7447-4_27
Pubmed ID
29116523
Book ISBNs
978-1-4939-7446-7, 978-1-4939-7447-4
Authors

Zantow, Jonas, Moreira, Gustavo Marçal Schmidt Garcia, Dübel, Stefan, Hust, Michael, Jonas Zantow, Gustavo Marçal Schmidt Garcia Moreira, Stefan Dübel, Michael Hust

Abstract

ORFeome phage display allows the efficient functional screening of entire proteomes or even metaproteomes to identify immunogenic proteins. For this purpose, randomly fragmented, whole genomes or metagenomes are cloned into a phage-display vector allowing positive selection for open reading frames (ORF) to improve the library quality. These libraries display all possible proteins encoded by a pathogen or a microbiome on the phage surface. Consequently, immunogenic proteins can be selected from these libraries using disease-related immunoglobulins from patient serum. ORFeome phage display in particular allows the identification of immunogenic proteins that are only expressed in the host-pathogen interaction but not in cultivation, as well as the detection of very low expressed and very small immunogens and immunogenic proteins of non-cultivable organisms. The identified immunogenic proteins are potential biomarkers for the development of diagnostic assays or vaccines. These articles will give an introduction to ORFeome phage-display technology and give detailed protocols to identify immunogenic proteins by phage display.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 30 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 20%
Student > Master 6 20%
Other 2 7%
Student > Doctoral Student 2 7%
Student > Ph. D. Student 2 7%
Other 2 7%
Unknown 10 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 23%
Agricultural and Biological Sciences 6 20%
Immunology and Microbiology 3 10%
Chemistry 2 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 2 7%
Unknown 9 30%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2017.
All research outputs
#18,909,315
of 24,093,053 outputs
Outputs from Methods in molecular biology
#7,740
of 13,601 outputs
Outputs of similar age
#319,996
of 449,849 outputs
Outputs of similar age from Methods in molecular biology
#863
of 1,480 outputs
Altmetric has tracked 24,093,053 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,601 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 449,849 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,480 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.