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Phage Display

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Cover of 'Phage Display'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Construction of Human Immune and Naive scFv Libraries
  3. Altmetric Badge
    Chapter 2 Construction of Naive and Immune Human Fab Phage-Display Library
  4. Altmetric Badge
    Chapter 3 Construction of Synthetic Antibody Phage-Display Libraries
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    Chapter 4 Modular Construction of Large Non-Immune Human Antibody Phage-Display Libraries from Variable Heavy and Light Chain Gene Cassettes
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    Chapter 5 Construction of Macaque Immune-Libraries
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    Chapter 6 Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format
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    Chapter 7 Construction of Rabbit Immune Antibody Libraries
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    Chapter 8 Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries
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    Chapter 9 Construction of High-Quality Camel Immune Antibody Libraries
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    Chapter 10 Construction of Chicken Antibody Libraries
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    Chapter 11 Construction and Selection of Affilin® Phage Display Libraries
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    Chapter 12 Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies
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    Chapter 13 Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach
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    Chapter 14 Parallelized Antibody Selection in Microtiter Plates
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    Chapter 15 Mass Spectrometry Immuno Assay (MSIA™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning
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    Chapter 16 Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection
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    Chapter 17 Phage Display and Selections on Cells
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    Chapter 18 Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens
  20. Altmetric Badge
    Chapter 19 Antibody Isolation From a Human Synthetic Combinatorial and Other Libraries of Single-Chain Antibodies
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    Chapter 20 Screening Phage-Display Antibody Libraries Using Protein Arrays
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    Chapter 21 Antibody Selection on FFPE Tissue Slides
  23. Altmetric Badge
    Chapter 22 Antibody Affinity and Stability Maturation by Error-Prone PCR
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    Chapter 23 Upgrading Affinity Screening Experiments by Analysis of Next-Generation Sequencing Data
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    Chapter 24 Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Discovery
  26. Altmetric Badge
    Chapter 25 High-Throughput IgG Reformatting and Expression
  27. Altmetric Badge
    Chapter 26 Monitoring Phage Biopanning by Next-Generation Sequencing
  28. Altmetric Badge
    Chapter 27 ORFeome Phage Display.
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    Chapter 28 Epitope Mapping by Phage Display
  30. Altmetric Badge
    Chapter 29 Metasecretome Phage Display
  31. Altmetric Badge
    Chapter 30 Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages
Attention for Chapter 26: Monitoring Phage Biopanning by Next-Generation Sequencing
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Chapter title
Monitoring Phage Biopanning by Next-Generation Sequencing
Chapter number 26
Book title
Phage Display
Published in
Methods in molecular biology, November 2017
DOI 10.1007/978-1-4939-7447-4_26
Pubmed ID
Book ISBNs
978-1-4939-7446-7, 978-1-4939-7447-4
Authors

Vaisman-Mentesh, Anna, Wine, Yariv, Anna Vaisman-Mentesh, Yariv Wine

Abstract

Phage display has enabled the rapid isolation of antigen-specific antibodies from combinatorial libraries of the variable heavy chain gene (VH) and variable light chain gene (VL). The method is based on genetic engineering of bacteriophages and repeated rounds of antigen-guided selection by phage biopanning.Next-Generation Sequencing (NGS) coupled with bioinformatics are powerful tools for analyzing the large number of DNA sequences present in an immune library.Here, we describe a method that demonstrates how NGS analysis enhances phage biopanning of complex antibody libraries as well as facilitates the antibody discovery process.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 23%
Researcher 3 14%
Other 2 9%
Student > Ph. D. Student 2 9%
Student > Bachelor 2 9%
Other 2 9%
Unknown 6 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 27%
Agricultural and Biological Sciences 3 14%
Veterinary Science and Veterinary Medicine 1 5%
Arts and Humanities 1 5%
Computer Science 1 5%
Other 2 9%
Unknown 8 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 July 2018.
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#18,576,001
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Outputs from Methods in molecular biology
#7,962
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#253,961
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Outputs of similar age from Methods in molecular biology
#33
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