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Mendeley readers
| Chapter title |
Phage Display and Selections on Cells
|
|---|---|
| Chapter number | 17 |
| Book title |
Phage Display
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7447-4_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7446-7, 978-1-4939-7447-4
|
| Authors |
Wieland Fahr, André Frenzel |
| Abstract |
Antibody identification by phage display on protein or peptide targets is well established and many protocols are available. But there are many targets that cannot be expressed recombinantly or, like peptides, do not reflect correct folding of the protein. Most of these targets are cell surface receptors. Here, we describe a protocol for a panning strategy on cells to obtain specific binders to cell surface receptors. A depletion step is included to prevent enrichment of antibodies that bind to unwanted targets. Each step of the protocol is explained and variations of this protocol are given. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 21% |
| Student > Ph. D. Student | 3 | 16% |
| Other | 2 | 11% |
| Student > Postgraduate | 2 | 11% |
| Researcher | 2 | 11% |
| Other | 2 | 11% |
| Unknown | 4 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 42% |
| Agricultural and Biological Sciences | 3 | 16% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Chemistry | 1 | 5% |
| Engineering | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 5 | 26% |