| Chapter title |
Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach
|
|---|---|
| Chapter number | 13 |
| Book title |
Phage Display
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7447-4_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7446-7, 978-1-4939-7447-4
|
| Authors |
Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, Nai-Kong V. Cheung |
| Abstract |
Antibodies that bind peptide-MHC (pMHC) complex in a manner akin to T-cell receptor (TCR) have not only helped in understanding the mechanism of TCR-pMHC interactions in the context of T-cell biology, but also spurred considerable interest in recent years as potential cancer therapeutics. Traditional methods to generate such antibodies using hybridoma and B-cell sorting technologies are sometimes inadequate, possibly due to the small contribution of peptide to the overall B-cell epitope space on the surface of the pMHC complex (typical peptide MW = 1 kDa versus MHC MW = 45 kDa), and to the multiple efficiency limiting steps inherent in these methods. In this chapter, we describe a phage display approach for the rapid generation of such antibodies with high specificity and affinity. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 4 | 27% |
| Student > Doctoral Student | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Master | 2 | 13% |
| Student > Bachelor | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 40% |
| Agricultural and Biological Sciences | 2 | 13% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Medicine and Dentistry | 1 | 7% |
| Engineering | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 4 | 27% |