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Mendeley readers
Chapter title |
Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies
|
---|---|
Chapter number | 12 |
Book title |
Phage Display
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7447-4_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7446-7, 978-1-4939-7447-4
|
Authors |
Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau |
Abstract |
Libraries of antibody fragments displayed on filamentous phages have proved their value to generate human antibodies against virtually any target. We describe here a simple protocol to make large and diverse libraries based on a single or a limited number of frameworks. The approach is flexible enough to be used with any antibody format, either single-chain (scFv, VHH) or multi-chain (Fv, Fab, (Fab')2), and to target in a single step the six complementarity-determining regions-or any other part-of the antibody molecule. Using this protocol, libraries larger than 10(10) can be easily constructed in a single week. |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 48% |
Student > Master | 3 | 13% |
Other | 2 | 9% |
Student > Ph. D. Student | 2 | 9% |
Student > Doctoral Student | 2 | 9% |
Other | 2 | 9% |
Unknown | 1 | 4% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 39% |
Immunology and Microbiology | 5 | 22% |
Agricultural and Biological Sciences | 2 | 9% |
Nursing and Health Professions | 1 | 4% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Other | 2 | 9% |
Unknown | 3 | 13% |