Next Generation Sequencing

Chapter 1 An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome

Chapter 2 Measuring Nascent Transcripts by Nascent-seq

Chapter 3 Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis

Chapter 4 Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure

Chapter 5 Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing

Chapter 6 ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras

Chapter 7 Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach

Chapter 8 Sequencing of Genomes from Environmental Single Cells

Chapter 9 SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms

Chapter 10 CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers

Chapter 11 Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities

Chapter 12 A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures

Chapter 13 Next-Generation Sequencing of Genome-Wide CRISPR Screens

Chapter 14 Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells

Chapter 15 Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells

Chapter 16 Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites