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Next Generation Sequencing

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Cover of 'Next Generation Sequencing'

Table of Contents

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    Book Overview
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    Chapter 1 An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome
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    Chapter 2 Measuring Nascent Transcripts by Nascent-seq
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    Chapter 3 Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
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    Chapter 4 Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure
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    Chapter 5 Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing
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    Chapter 6 ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras
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    Chapter 7 Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach
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    Chapter 8 Sequencing of Genomes from Environmental Single Cells
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    Chapter 9 SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms
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    Chapter 10 CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers
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    Chapter 11 Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities
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    Chapter 12 A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures
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    Chapter 13 Next-Generation Sequencing of Genome-Wide CRISPR Screens
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    Chapter 14 Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells
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    Chapter 15 Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
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    Chapter 16 Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites
Attention for Chapter 15: Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
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Chapter title
Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
Chapter number 15
Book title
Next Generation Sequencing
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7514-3_15
Pubmed ID
Book ISBNs
978-1-4939-7512-9, 978-1-4939-7514-3
Authors

Xiangzhi Meng, Nicole Riley, Ryan Thompson, Siddhartha Sharma

Abstract

Hi-C is a methodology developed to reveal chromosomal interactions from a genome-wide perspective. Here, we described a protocol for generating Hi-C sequencing libraries in resting and activated human naive CD4 T cells to investigate activation-induced chromatin structure re-arrangement in T cell activation followed by a section reviewing the general concepts of Hi-C data analysis.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 30%
Other 2 20%
Student > Ph. D. Student 1 10%
Student > Master 1 10%
Unknown 3 30%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 30%
Agricultural and Biological Sciences 2 20%
Arts and Humanities 1 10%
Unknown 4 40%