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Next Generation Sequencing

Overview of attention for book
Cover of 'Next Generation Sequencing'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 An Integrated Polysome Profiling and Ribosome Profiling Method to Investigate In Vivo Translatome
  3. Altmetric Badge
    Chapter 2 Measuring Nascent Transcripts by Nascent-seq
  4. Altmetric Badge
    Chapter 3 Genome-Wide Copy Number Alteration Detection in Preimplantation Genetic Diagnosis
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    Chapter 4 Multiplexed Targeted Sequencing for Oxford Nanopore MinION: A Detailed Library Preparation Procedure
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    Chapter 5 Hi-Plex for Simple, Accurate, and Cost-Effective Amplicon-based Targeted DNA Sequencing
  7. Altmetric Badge
    Chapter 6 ClickSeq: Replacing Fragmentation and Enzymatic Ligation with Click-Chemistry to Prevent Sequence Chimeras
  8. Altmetric Badge
    Chapter 7 Genome-Wide Analysis of DNA Methylation in Single Cells Using a Post-bisulfite Adapter Tagging Approach
  9. Altmetric Badge
    Chapter 8 Sequencing of Genomes from Environmental Single Cells
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    Chapter 9 SNP Discovery from Single and Multiplex Genome Assemblies of Non-model Organisms
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    Chapter 10 CleanTag Adapters Improve Small RNA Next-Generation Sequencing Library Preparation by Reducing Adapter Dimers
  12. Altmetric Badge
    Chapter 11 Sampling, Extraction, and High-Throughput Sequencing Methods for Environmental Microbial and Viral Communities
  13. Altmetric Badge
    Chapter 12 A Bloody Primer: Analysis of RNA-Seq from Tissue Admixtures
  14. Altmetric Badge
    Chapter 13 Next-Generation Sequencing of Genome-Wide CRISPR Screens
  15. Altmetric Badge
    Chapter 14 Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells
  16. Altmetric Badge
    Chapter 15 Investigate Global Chromosomal Interaction by Hi-C in Human Naive CD4 T Cells
  17. Altmetric Badge
    Chapter 16 Primer Extension, Capture, and On-Bead cDNA Ligation: An Efficient RNAseq Library Prep Method for Determining Reverse Transcription Termination Sites
Attention for Chapter 2: Measuring Nascent Transcripts by Nascent-seq
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Chapter title
Measuring Nascent Transcripts by Nascent-seq
Chapter number 2
Book title
Next Generation Sequencing
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7514-3_2
Pubmed ID
Book ISBNs
978-1-4939-7512-9, 978-1-4939-7514-3
Authors

Fei Xavier Chen, Stacy A. Marshall, Yu Deng, Sun Tianjiao

Abstract

A complete understanding of transcription and co-transcriptional RNA processing events by polymerase requires precise and robust approaches to visualize polymerase progress and quantify nascent transcripts on a genome-wide scale. Here, we present a transcriptome-wide method to measure the level of nascent transcribing RNA in a fast and unbiased manner.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Unspecified 1 13%
Researcher 1 13%
Unknown 4 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 25%
Unspecified 1 13%
Immunology and Microbiology 1 13%
Unknown 4 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 July 2018.
All research outputs
#14,086,689
of 23,011,300 outputs
Outputs from Methods in molecular biology
#3,965
of 13,157 outputs
Outputs of similar age
#232,652
of 442,319 outputs
Outputs of similar age from Methods in molecular biology
#395
of 1,498 outputs
Altmetric has tracked 23,011,300 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,157 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 68% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 442,319 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,498 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.