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Mendeley readers
Chapter title |
Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm
|
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Chapter number | 7 |
Book title |
Proteases and Cancer
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7595-2_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7594-5, 978-1-4939-7595-2
|
Authors |
Dong Hyun Nam, Ki Baek Lee, Xin Ge |
Abstract |
Due to their central roles in tumor growth and invasion, milligram-level amounts of active MMPs are frequently required for cancer research and development of chemical or biological MMP inhibitors. Here we describe methods for functional production of catalytic domains of MMPs (cdMMPs) in E. coli periplasm without refolding or activation process. We demonstrate applications of this straightforward approach for cdMMP-9, cdMMP-14, and cdMMP-14 mutants. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 29% |
Student > Bachelor | 1 | 14% |
Other | 1 | 14% |
Student > Master | 1 | 14% |
Researcher | 1 | 14% |
Other | 0 | 0% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 71% |
Chemical Engineering | 1 | 14% |
Unknown | 1 | 14% |