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Proteases and Cancer

Overview of attention for book
Cover of 'Proteases and Cancer'

Table of Contents

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    Book Overview
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    Chapter 1 Dissecting Degradomes: Analysis of Protease-Coding Genes
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    Chapter 2 Identification of Protease Cleavage Sites and Substrates in Cancer by Carboxy-TAILS (C-TAILS)
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    Chapter 3 Identification of Proteolytic Cleavage Sites of EphA2 by Membrane Type 1 Matrix Metalloproteinase on the Surface of Cancer Cells
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    Chapter 4 Biotin-Chasing Assay to Evaluate uPAR Stability and Cleavage on the Surface of Cells
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    Chapter 5 Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease
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    Chapter 6 A Simple Cell-Based Assay for the Detection of Surface Protein Shedding by Rhomboid Proteases
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    Chapter 7 Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm
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    Chapter 8 Autophagy and Proteases: Basic Study of the Autophagic Flux by Western Blot
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    Chapter 9 Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture
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    Chapter 10 Analysis of Enzymatic Activity of Matrix Metalloproteinase (MMP) by Collagen Zymography in Melanoma
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    Chapter 11 Measurement of Protease Activities Using Fluorogenic Substrates
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    Chapter 12 Targeting the Expression of Cathepsin B Using CRISPR/Cas9 System in Mammalian Cancer Cells
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    Chapter 13 Assessing the Influence of a Protease in Cell Migration Using the Barrier-Migration Assay
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    Chapter 14 Analysis of Invasive Activity of CAF Spheroids into Three Dimensional (3D) Collagen Matrices
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    Chapter 15 Beyond the Proteolytic Activity: Examining the Functional Relevance of the Ancillary Domains Using Tri-Dimensional (3D) Spheroid Invasion Assay
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    Chapter 16 Analyzing the Type II Transmembrane Serine Protease Hepsin-Dependent Basement Membrane Remodeling in 3D Cell Culture
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    Chapter 17 Evaluation of Tumor Vasculature Using a Syngeneic Tumor Model in Wild-Type and Genetically Modified Mice
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    Chapter 18 3D Image Analysis of the Microvasculature in Healthy and Diseased Tissues
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    Chapter 19 Human Tumor Tissue-Based 3D In Vitro Invasion Assays
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    Chapter 20 Ear Sponge Assay: A Method to Investigate Angiogenesis and Lymphangiogenesis in Mice
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    Chapter 21 Cancer Susceptibility Models in Protease-Deficient Mice
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    Chapter 22 Imaging Proteolytic Activities in Mouse Models of Cancer
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    Chapter 23 Protease Silencing to Explore the Molecular Mechanisms of Cancer and Aging
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    Chapter 24 Analysis of Somatic DNA Methylation Alterations of Genes Encoding Cell Surface Metallopeptidases in Colorectal Cancer
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    Chapter 25 Targeting Deubiquitinases in Cancer
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    Chapter 26 Generation of Highly Selective MMP Antibody Inhibitors
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    Chapter 27 Strategies to Target Matrix Metalloproteinases as Therapeutic Approach in Cancer
Attention for Chapter 9: Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture
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Chapter title
Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture
Chapter number 9
Book title
Proteases and Cancer
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7595-2_9
Pubmed ID
29318546
Book ISBNs
978-1-4939-7594-5, 978-1-4939-7595-2
Authors

Aastha Chhabra, Vibha Rani

Abstract

Gelatin zymography, first described by Heussen and Dowdle in the 1980s, is a widely used valuable tool in research and diagnostics. The technique identifies gelatinases by the degradation of their preferential substrate as well as by their molecular weight (kDa). We here describe detailed methodology for the detection of pro- and active- forms of both MMP-2 (gelatinase A) and MMP-9 (gelatinase B) in cells using norepinephrine-stimulated H9c2 cardiomyoblasts as model. An easy to follow step-by-step protocol has been carefully written for reliable results. We also suggest an acceptable method for quantification of gelatin zymograms.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 3 17%
Student > Bachelor 3 17%
Student > Master 3 17%
Student > Ph. D. Student 2 11%
Researcher 2 11%
Other 0 0%
Unknown 5 28%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 22%
Agricultural and Biological Sciences 4 22%
Chemistry 2 11%
Earth and Planetary Sciences 1 6%
Medicine and Dentistry 1 6%
Other 1 6%
Unknown 5 28%

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