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Mendeley readers
Chapter title |
Measurement of Protease Activities Using Fluorogenic Substrates
|
---|---|
Chapter number | 11 |
Book title |
Proteases and Cancer
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7595-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7594-5, 978-1-4939-7595-2
|
Authors |
Salvatore Santamaria, Hideaki Nagase, Santamaria, Salvatore, Nagase, Hideaki |
Abstract |
Matrix metalloproteinases and the related metalloproteases are implicated in cancer progression. They are endopeptidases that require several defined amino acid residues in both N-terminal and C-terminal sides of the scissile bond. Fluorogenic Förster resonance energy transfer (FRET) substrates that harbor a fluorophore and a quencher on opposite sides of the scissile bond are conveniently used to measure their activities. In this chapter, we describe the principle of FRET substrates and how to use them to measure activities and kinetic parameters of endopeptidases. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 38% |
Student > Bachelor | 1 | 13% |
Librarian | 1 | 13% |
Unknown | 3 | 38% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Computer Science | 1 | 13% |
Chemistry | 1 | 13% |
Unknown | 4 | 50% |