Chapter title |
Study of Telomere Dysfunction in TP53 Mutant LoVo Cell Lines as a Model for Genomic Instability
|
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Chapter number | 14 |
Book title |
Chromothripsis
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Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7780-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7779-6, 978-1-4939-7780-2
|
Authors |
Oumar Samassekou, Nathalie Bastien, Ju Yan, Sabine Mai, Régen Drouin, Samassekou, Oumar, Bastien, Nathalie, Yan, Ju, Mai, Sabine, Drouin, Régen |
Abstract |
Telomere restriction fragment, 3D quantitative FISH on nuclei, and quantitative FISH on metaphases are complementary approaches that explore telomere dysfunction genomically, cellularly, and chromosomally, respectively. We used these approaches to study association between telomere dysfunction and degree of genomic instability related to TP53 mutations in LoVo isogenic cell lines. We found a strong correlation between degree of genomic instability, telomere dysfunction, and specific mutations of TP53. The use of complementary approaches to study telomere biology is essential to have a comprehensive understanding of telomere involvement in genomic instability. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 33% |
Student > Doctoral Student | 2 | 22% |
Researcher | 2 | 22% |
Student > Master | 1 | 11% |
Professor > Associate Professor | 1 | 11% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 44% |
Agricultural and Biological Sciences | 2 | 22% |
Medicine and Dentistry | 2 | 22% |
Unknown | 1 | 11% |