Chapter title |
Live-Cell Fluorescence Imaging for Phenotypic Analysis of Mitosis.
|
---|---|
Chapter number | 31 |
Book title |
Cell Cycle Control
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-0888-2_31 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0887-5, 978-1-4939-0888-2
|
Authors |
Sushama Sivakumar, John R Daum, Gary J Gorbsky, John R. Daum, Gary J. Gorbsky, Sivakumar, Sushama, Daum, John R., Gorbsky, Gary J. |
Abstract |
Live-cell fluorescence microscopy is a powerful tool for characterizing aberrant mitotic phenotypes resulting from exposure to chemical inhibitors or after depletion of protein targets by RNA interference or other methods. Live imaging of cultured cells during mitotic progression presents challenges in maintaining optimal health of cells while achieving the temporal and spatial resolution to accomplish the goals of the study. We describe herein strategies to monitor and analyze mammalian cell mitosis with standard, inverted, fluorescence microscopy systems that are widely available. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 3% |
Unknown | 33 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 7 | 21% |
Student > Ph. D. Student | 5 | 15% |
Student > Bachelor | 4 | 12% |
Researcher | 3 | 9% |
Student > Doctoral Student | 2 | 6% |
Other | 5 | 15% |
Unknown | 8 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 15 | 44% |
Agricultural and Biological Sciences | 6 | 18% |
Computer Science | 2 | 6% |
Medicine and Dentistry | 1 | 3% |
Chemistry | 1 | 3% |
Other | 0 | 0% |
Unknown | 9 | 26% |