Chapter title |
Analyzing sister chromatid cohesion in Mammalian cells.
|
---|---|
Chapter number | 32 |
Book title |
Cell Cycle Control
|
Published in |
Methods in molecular biology, June 2014
|
DOI | 10.1007/978-1-4939-0888-2_32 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0887-5, 978-1-4939-0888-2
|
Authors |
Feeney KM, McFarlane-Majeed L, Parish JL, Katherine M. Feeney, Laura McFarlane-Majeed, Joanna L. Parish |
Abstract |
The metaphase chromosome spread technique and subsequent analysis of sister chromatid cohesion is used for (clinical) diagnosis of genetic abnormalities that can cause aberrant sister chromatid cohesion. In addition, the technique can be used to assess the contribution of novel genes to the cohesion establishment and maintenance pathways. Cells are swelled in a hypotonic solution and fixed in Carnoy's solution. Samples are then dropped onto glass slides, and the spread chromosomes are stained and visualized by microscopy. Defects in sister chromatid cohesion can be easily assessed using this method, examples of which are given. |
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