Chapter title |
Tracking Histone Variant Nucleosomes Across the Human Cell Cycle Using Biophysical, Biochemical, and Cytological Analyses.
|
---|---|
Chapter number | 34 |
Book title |
Cell Cycle Control
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-0888-2_34 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0887-5, 978-1-4939-0888-2
|
Authors |
Marcin P Walkiewicz, Minh Bui, Delphine Quénet, Yamini Dalal, Walkiewicz MP, Bui M, Quénet D, Dalal Y, Marcin P. Walkiewicz, Walkiewicz, Marcin P., Bui, Minh, Quénet, Delphine, Dalal, Yamini |
Abstract |
Histone variants such as H3.3, macroH2A, H2A.Z, and CENP-A are important epigenetic modifiers of the chromatin state in eukaryotic genomes. The centromeric histone H3 variant CENP-A/CENH3 epigenetically marks centromeres and is required for assembly of the kinetochore complex, a region of the chromosome that is responsible for proper genome segregation during mitosis. Several diverse techniques using biochemical, cell biology, and biophysical approaches have been utilized to study the nature of the CENP-A nucleosome across the cell cycle. In this chapter, we describe methods for CENP-A nucleosome purification and separation of CENP-A from other core histones using traditional SDS-PAGE and more resolving techniques such as Triton acid urea (TAU) and two-dimensional gels. We also discuss methods for observation of CENP-A on chromatin fibers using immunofluorescence. Finally, we provide a detailed description of analysis of chromatin structures using atomic force microscopy. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 29% |
Student > Bachelor | 1 | 14% |
Other | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Professor > Associate Professor | 1 | 14% |
Other | 0 | 0% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 57% |
Agricultural and Biological Sciences | 1 | 14% |
Neuroscience | 1 | 14% |
Unknown | 1 | 14% |