Chapter title |
Histone Deacetylase Activity Assay
|
---|---|
Chapter number | 7 |
Book title |
Chromatin Protocols
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2474-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2473-8, 978-1-4939-2474-5
|
Authors |
Lirong Peng, Zhigang Yuan, Edward Seto |
Abstract |
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ε-amino groups of conserved lysine residues in the amino terminal tail of histones. Accumulating evidence suggests that many, if not all, HDACs can also deacetylate nonhistone proteins. Through deacetylating histones and nonhistone proteins, HDACs regulate a variety of cellular processes including gene transcription, cell differentiation, DNA damage responses, and apoptosis. Aberrant HDACs are implicated in many human diseases and, therefore, it is important to have a consistent and reliable assay for analyzing HDAC activities. The focus of this chapter is to provide up-to-date, easy-to-follow, approaches and techniques, for the assay of HDAC enzymatic activities. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 5 | 31% |
Student > Ph. D. Student | 3 | 19% |
Student > Bachelor | 1 | 6% |
Other | 1 | 6% |
Researcher | 1 | 6% |
Other | 0 | 0% |
Unknown | 5 | 31% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 25% |
Agricultural and Biological Sciences | 4 | 25% |
Chemistry | 2 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Unknown | 5 | 31% |