| Chapter title |
In vitro replication assay with Mammalian cell extracts.
|
|---|---|
| Chapter number | 20 |
| Book title |
Chromatin Protocols
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2474-5_20 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2473-8, 978-1-4939-2474-5
|
| Authors |
Rizwani, Wasia, Chellappan, Srikumar P, Wasia Rizwani, Srikumar P. Chellappan |
| Abstract |
Regulatory mechanisms are crucial to control DNA replication during cell cycle in eukaryotic cells. Cell-free in vitro replication assay (IVRA) is one of the widely used assays to understand the complex mammalian replication system. IVRA can provide a snapshot of the regulatory mechanisms controlling replication in higher eukaryotes by using a single plasmid, pEPI-1. This chapter outlines the general strategies and protocols used to perform IVRA to study the differential recruitment of replication factors either independently or in combination, based on the experience in studying the role of prohibitin in replication as well as other published protocols. This method can be employed to identify not only proteins that assist replication but also proteins that inhibit replication of mammalian genome. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 33% |
| Researcher | 2 | 33% |
| Professor | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 50% |
| Agricultural and Biological Sciences | 1 | 17% |
| Immunology and Microbiology | 1 | 17% |
| Unknown | 1 | 17% |