You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
Chapter title |
Expression and Purification of Recombinant CDKs: CDK7, CDK8, and CDK9
|
---|---|
Chapter number | 3 |
Book title |
Cyclin-Dependent Kinase (CDK) Inhibitors
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-2926-9_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2925-2, 978-1-4939-2926-9
|
Authors |
Reena Pinhero, Krassimir Yankulov |
Abstract |
Cyclin-dependent kinases have established roles in the regulation of cell cycle, in gene expression and in cell differentiation. Many of these kinases have been considered as drug targets and numerous efforts have been made to develop specific and potent inhibitors against them. The first step in all of these attempts and in many other biochemical analyses is the production of highly purified and reliable kinase, most frequently in a recombinant form. In this chapter we describe our experience in the cloning, expression, and purification of CDKs using CDK7/CycH, CDK8/CycC, and CDK9/CycT1 as an example. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 50% |
Student > Ph. D. Student | 1 | 17% |
Unknown | 2 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Chemistry | 1 | 17% |
Unknown | 3 | 50% |