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Immunosenescence

Overview of attention for book
Cover of 'Immunosenescence'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation
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    Chapter 2 Flow Cytometry Analysis of NK Cell Phenotype and Function in Aging.
  4. Altmetric Badge
    Chapter 3 Flow Cytometric Identification of Fibrocytes in the Human Circulation.
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    Chapter 4 Experimental Approaches to Tissue Injury and Repair in Advanced Age.
  6. Altmetric Badge
    Chapter 5 Multicolor Digital Flow Cytometry in Human Translational Immunology
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    Chapter 6 Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates.
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    Chapter 7 Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry.
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    Chapter 8 Imaging Immunosenescence.
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    Chapter 9 Activation-Induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine
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    Chapter 10 Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry
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    Chapter 11 Immunosenecence
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    Chapter 12 Assays for Monitoring Macroautophagy Activity in T cells.
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    Chapter 13 Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides, and Protein Conformation in Biological Tissues
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    Chapter 14 Monitoring the DNA Damage Response at Dysfunctional Telomeres
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    Chapter 15 Single-Cell Analysis of T-Cell Receptor αβ Repertoire.
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    Chapter 16 Immunosenecence
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    Chapter 17 Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-Throughput DNA Sequencing.
  19. Altmetric Badge
    Chapter 18 Discovery of Novel microRNAs in Aging Caenorhabditis elegans.
  20. Altmetric Badge
    Chapter 19 Analysis of DNA Methylation by Pyrosequencing.
  21. Altmetric Badge
    Chapter 20 ERRATUM
Attention for Chapter 19: Analysis of DNA Methylation by Pyrosequencing.
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  • Good Attention Score compared to outputs of the same age and source (65th percentile)

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Citations

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Chapter title
Analysis of DNA Methylation by Pyrosequencing.
Chapter number 19
Book title
Immunosenescence
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2963-4_19
Pubmed ID
26420722
Book ISBNs
978-1-4939-2962-7, 978-1-4939-2963-4
Authors

Delaney, Colin, Garg, Sanjay K, Yung, Raymond, Colin Delaney, Sanjay K. Garg, Raymond Yung, Garg, Sanjay K.

Abstract

Pyrosequencing is a technique that uses a sequencing-by-synthesis system which is designed to quantify single-nucleotide polymorphisms (SNPs). Artificial C/T SNP creation via bisulfite modification permits measurement of DNA methylation locally and globally in real time. Alteration in DNA methylation has been implicated in aging, as well as aging-related conditions such as cancer, as well as cardiovascular, neurodegenerative, and autoimmune diseases. Considering its ubiquitous presence in divergent clinical pathologies, quantitative analysis of DNA CpG methylation both globally and at individual genes helps to elucidate the regulation of genes involved in pathophysiological conditions. The ability to detect and quantify the methylation pattern of DNA has the potential to serve as an early detection marker and potential drug target for several diseases. Here, we provide a detailed technical protocol for pyrosequencing supplemented by critical information about assay design and nuances of the system that provides a strong foundation for beginners in the field.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 229 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 <1%
Unknown 228 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 48 21%
Student > Bachelor 34 15%
Student > Master 29 13%
Student > Doctoral Student 14 6%
Researcher 13 6%
Other 24 10%
Unknown 67 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 71 31%
Agricultural and Biological Sciences 30 13%
Medicine and Dentistry 14 6%
Neuroscience 10 4%
Immunology and Microbiology 4 2%
Other 24 10%
Unknown 76 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 July 2016.
All research outputs
#15,153,715
of 23,306,612 outputs
Outputs from Methods in molecular biology
#4,838
of 13,326 outputs
Outputs of similar age
#200,914
of 355,666 outputs
Outputs of similar age from Methods in molecular biology
#304
of 1,004 outputs
Altmetric has tracked 23,306,612 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,326 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 355,666 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,004 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.

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