Chapter title |
Flow Cytometry Analysis of NK Cell Phenotype and Function in Aging.
|
---|---|
Chapter number | 2 |
Book title |
Immunosenescence
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2963-4_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2962-7, 978-1-4939-2963-4
|
Authors |
Tarazona, Raquel, Campos, Carmen, Pera, Alejandra, Sanchez-Correa, Beatriz, Solana, Rafael, Raquel Tarazona, Carmen Campos, Alejandra Pera, Beatriz Sanchez-Correa, Rafael Solana |
Abstract |
Natural killer (NK) cells represent a subpopulation of lymphocytes involved in innate immunity, defined recently as group 1 of innate lymphoid cells (ILCs). NK cells are cytotoxic lymphocytes with a relevant role in the destruction of transformed cells as virus-infected or tumor cells, as well as the regulation of the immune response through cytokine and chemokine production that activates other cellular components of innate and adaptive immunity. In humans, NK cell subsets have been defined according to the level of expression of CD56. Aging differentially affects NK cell subsets and NK cell function. Here, we describe protocols for the delineation of NK cell subsets and the analysis of their functional capacity using multiparametric flow cytometry. |
X Demographics
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
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Student > Master | 4 | 20% |
Student > Bachelor | 2 | 10% |
Professor | 2 | 10% |
Student > Ph. D. Student | 2 | 10% |
Student > Postgraduate | 2 | 10% |
Other | 3 | 15% |
Unknown | 5 | 25% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 5 | 25% |
Biochemistry, Genetics and Molecular Biology | 4 | 20% |
Agricultural and Biological Sciences | 2 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Nursing and Health Professions | 1 | 5% |
Other | 3 | 15% |
Unknown | 4 | 20% |