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Plant Cytogenetics

Overview of attention for book
Attention for Chapter 2: Chromosome Painting by GISH and Multicolor FISH
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Chapter title
Chromosome Painting by GISH and Multicolor FISH
Chapter number 2
Book title
Plant Cytogenetics
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3622-9_2
Pubmed ID
Book ISBNs
978-1-4939-3620-5, 978-1-4939-3622-9
Authors

Steven S. Xu, Zhao Liu, Qijun Zhang, Zhixia Niu, Chao-Chien Jan, Xiwen Cai

Abstract

Fluorescent in situ hybridization (FISH) is a powerful cytogenetic technique for identifying chromosomes and mapping specific genes and DNA sequences on individual chromosomes. Genomic in situ hybridization (GISH) and multicolor FISH (mc-FISH) represent two special types of FISH techniques. Both GISH and mc-FISH experiments have general steps and features of FISH, including chromosome preparation, probe labeling, blocking DNA preparation, target-probe DNA hybridization, post-hybridization washes, and hybridization signal detection. Specifically, GISH uses total genomic DNA from two species as probe and blocking DNA, respectively, and it can differentiate chromosomes from different genomes. The mc-FISH takes advantage of simultaneous hybridization of several DNA probes labeled by different fluorochromes to different targets on the same chromosome sample. Hybridization signals from different probes are detected using different fluorescence filter sets. Multicolor FISH can provide more structural details for target chromosomes than single-color FISH. In this chapter, we present the general experimental procedures for these two techniques with specific details in the critical steps we have modified in our laboratories.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 45%
Student > Ph. D. Student 3 27%
Researcher 1 9%
Student > Doctoral Student 1 9%
Unknown 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 55%
Biochemistry, Genetics and Molecular Biology 3 27%
Neuroscience 1 9%
Unknown 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#10,104,440
of 12,632,374 outputs
Outputs from Methods in molecular biology
#4,278
of 8,351 outputs
Outputs of similar age
#201,020
of 273,696 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
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