Chapter title |
Use of cocultures for the study of cellular interactions influencing B cell regulatory functions.
|
---|---|
Chapter number | 12 |
Book title |
Regulatory B Cells
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1161-5_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1160-8, 978-1-4939-1161-5
|
Authors |
Francesca Mion, Federica Benvenuti, Carlo Pucillo, Gaetano Vitale |
Abstract |
Many immunological processes are contextually controlled by complex interactions among different cell types. Several studies have shown that B cells produce the immune regulatory cytokine IL-10 in response to different external stimuli but also to immune-mediated signals. Endogenous signals that derive from the cross talk between B lymphocytes and other cells of the immune system can affect IL-10 production by B cells in both physiological and pathological conditions. With the aim to provide a guide for the study of how partner cells can induce IL-10-producing B cells, here we describe the protocols to investigate IL-10 production at a single cell level in a dendritic cell-B cell coculture in vitro system. These methods are a proof of concept that can be easily extrapolated and adapted to the study of the interaction between B cells and other immune cell types. |
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