Chapter title |
Generation and identification of tumor-evoked regulatory B cells.
|
---|---|
Chapter number | 19 |
Book title |
Regulatory B Cells
|
Published in |
Methods in molecular biology, July 2014
|
DOI | 10.1007/978-1-4939-1161-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1160-8, 978-1-4939-1161-5
|
Authors |
Biragyn A, Lee-Chang C, Bodogai M, Arya Biragyn, Catalina Lee-Chang, Monica Bodogai |
Abstract |
The involvement of Bregs in cancer remains poorly understood despite their well-documented regulation of responses to the self and protection from harmful autoimmunity. We recently discovered a unique regulatory B cell subset evoked by breast cancer to mediate protection of metastasizing cancer cells. These results together with the wealth of findings of the last 40 years on B cells in tumorigenesis suggest the existence of additional cancer Bregs modulating anticancer responses. To facilitate the search for them, here we provide our detailed protocol for the characterization and generation of tumor-evoked regulatory B cells. Wherever applicable, we also discuss nuances and uniqueness of a Breg study in cancer to warn potential pitfalls. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 43% |
Student > Bachelor | 1 | 14% |
Student > Ph. D. Student | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 29% |
Immunology and Microbiology | 1 | 14% |
Biochemistry, Genetics and Molecular Biology | 1 | 14% |
Unknown | 3 | 43% |