Chapter title |
Characterization and Activity of Fas Ligand Producing CD5(+) B Cells.
|
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Chapter number | 7 |
Book title |
Regulatory B Cells
|
Published in |
Methods in molecular biology, July 2014
|
DOI | 10.1007/978-1-4939-1161-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1160-8, 978-1-4939-1161-5
|
Authors |
Lundy SK, Klinker MW, Steven K. Lundy, Matthew W. Klinker, Lundy, Steven K., Klinker, Matthew W. |
Abstract |
B lymphocytes make several contributions to immune regulation including production of antibodies with regulatory properties, release of immune suppressive cytokines, and expression of death-inducing ligands. A role for Fas ligand (FasL)-expressing "killer" B cells in regulating T helper cell survival and chronic inflammation has been demonstrated in animal models of schistosome worm infection, asthma, and autoimmune arthritis. Interestingly, a population of CD5(+) B cells found in the spleen and lungs of naïve mice constitutively expresses FasL and has potent killer function against T helper cells that is antigen-specific and FasL-dependent. Killer B cells therefore represent a novel target for immune modulation in many disease settings. Our laboratory has recently published methods of characterizing FasL(+) B cells and inducing their proliferation in vitro. This chapter will describe detailed methods of identifying and expanding killer B cells from mice, detecting FasL expression in B cells, and performing functional killing assays against antigen-specific TH cells. |
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