Synthetic Biology
Springer New York
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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology
|
|---|---|
| Chapter number | 25 |
| Book title |
Synthetic Biology
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7795-6_25 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7794-9, 978-1-4939-7795-6
|
| Authors |
Hung-En Lai, Simon Moore, Karen Polizzi, Paul Freemont, Lai, Hung-En, Moore, Simon, Polizzi, Karen, Freemont, Paul |
| Abstract |
Development of advanced synthetic biology tools is always in demand since they act as a platform technology to enable rapid prototyping of biological constructs in a high-throughput manner. EcoFlex is a modular cloning (MoClo) kit for Escherichia coli and is based on the Golden Gate principles, whereby Type IIS restriction enzymes (BsaI, BsmBI, BpiI) are used to construct modular genetic elements (biological parts) in a bottom-up approach. Here, we describe a collection of plasmids that stores various biological parts including promoters, RBSs, terminators, ORFs, and destination vectors, each encoding compatible overhangs allowing hierarchical assembly into single transcription units or a full-length polycistronic operon or biosynthetic pathway. A secondary module cloning site is also available for pathway optimization, in order to limit library size if necessary. Here, we show the utility of EcoFlex using the violacein biosynthesis pathway as an example. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 111 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 111 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 27 | 24% |
| Student > Ph. D. Student | 26 | 23% |
| Student > Master | 14 | 13% |
| Student > Bachelor | 13 | 12% |
| Other | 3 | 3% |
| Other | 9 | 8% |
| Unknown | 19 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 57 | 51% |
| Agricultural and Biological Sciences | 14 | 13% |
| Engineering | 4 | 4% |
| Chemical Engineering | 3 | 3% |
| Chemistry | 3 | 3% |
| Other | 8 | 7% |
| Unknown | 22 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2018.
All research outputs
#15,708,425
of 23,344,526 outputs
Outputs from Methods in molecular biology
#5,497
of 13,338 outputs
Outputs of similar age
#271,760
of 444,166 outputs
Outputs of similar age from Methods in molecular biology
#600
of 1,502 outputs
Altmetric has tracked 23,344,526 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,338 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 444,166 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,502 others from the same source and published within six weeks on either side of this one. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.