Chapter title |
Methods for Generating Microbial Cocultures that Grow in the Absence of Fixed Carbon or Nitrogen
|
---|---|
Chapter number | 3 |
Book title |
Synthetic Biology
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7795-6_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7794-9, 978-1-4939-7795-6
|
Authors |
Matthew J. Smith, Matthew B. Francis |
Abstract |
This work is motivated by both (1) the desire to make interesting products without the reliance on fixed carbon or fixed nitrogen using microbial cocultures, and (2) the desire to develop new methods for growing microbial communities. The bioplastic polyhydroxybutyrate (PHB), for example, is considered prohibitively expensive to make from sugar (compared to polypropylene). Utilizing and building on engineered strains of Synechococcus elongatus and Azotobacter vinelandii, we have combined a nitrogen-fixing organism with a carbon-fixing organism to make PHB from air, water, sunlight, and trace minerals. Our observations of coculture growth in batch culture led us to develop an improved system based on manipulating the osmotic pressure within a hydrogel. The methods we used to develop this coculture are described in detail in the following chapter, including notes detailing some of our additional observations or thoughts on this system. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 18% |
Student > Ph. D. Student | 1 | 9% |
Student > Bachelor | 1 | 9% |
Professor > Associate Professor | 1 | 9% |
Unknown | 6 | 55% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 18% |
Agricultural and Biological Sciences | 2 | 18% |
Unknown | 7 | 64% |