| Chapter title |
Combining Membrane Potential Imaging with Other Optical Techniques.
|
|---|---|
| Chapter number | 4 |
| Book title |
Membrane Potential Imaging in the Nervous System and Heart
|
| Published in |
Advances in experimental medicine and biology, January 2015
|
| DOI | 10.1007/978-3-319-17641-3_4 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-917640-6, 978-3-31-917641-3
|
| Authors |
Jaafari, Nadia, Vogt, Kaspar E, Saggau, Peter, Leslie, Loew M, Zecevic, Dejan, Canepari, Marco, Nadia Jaafari, Kaspar E. Vogt, Peter Saggau, Loew M. Leslie, Dejan Zecevic, Marco Canepari, Vogt, Kaspar E., Leslie, Loew M. |
| Abstract |
Membrane potential imaging using voltage-sensitive dyes can be combined with other optical techniques for a variety of applications. Combining voltage imaging with Ca(2+) imaging allows correlating membrane potential changes with intracellular Ca(2+) signals or with Ca(2+) currents. Combining voltage imaging with uncaging techniques allows analyzing electrical signals elicited by photorelease of a particular molecule. This approach is also a useful tool to calibrate the change in fluorescence intensity in terms of membrane potential changes from different sites permitting spatial mapping of electrical activity. Finally, combining voltage imaging with optogenetics, in particular with channelrhodopsin stimulation, opens the gate to novel investigations of brain circuitries by allowing measurements of synaptic signals mediated by specific sets of neurons. Here we describe in detail the methods of membrane potential imaging in combination with other optical techniques and discus some important applications. |
X Demographics
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| Unknown | 1 | 100% |
Demographic breakdown
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| Members of the public | 1 | 100% |
Mendeley readers
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|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
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|---|---|---|
| Student > Ph. D. Student | 9 | 43% |
| Student > Bachelor | 3 | 14% |
| Student > Master | 2 | 10% |
| Professor > Associate Professor | 2 | 10% |
| Researcher | 2 | 10% |
| Other | 2 | 10% |
| Unknown | 1 | 5% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Chemistry | 2 | 10% |
| Medicine and Dentistry | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 2 | 10% |