Chapter title |
MicroRNA Expression and Regulation During Maize Somatic Embryogenesis
|
---|---|
Chapter number | 28 |
Book title |
Plant Cell Culture Protocols
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8594-4_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8593-7, 978-1-4939-8594-4
|
Authors |
Brenda Anabel López-Ruiz, Vasti Thamara Juárez-González, Elva Carolina Chávez-Hernández, Tzvetanka D. Dinkova, López-Ruiz, Brenda Anabel, Juárez-González, Vasti Thamara, Chávez-Hernández, Elva Carolina, Dinkova, Tzvetanka D. |
Abstract |
MicroRNAs are tiny molecules that strikingly change their expression patterns and distribution during somatic embryogenesis induction and plant regeneration. It is of great relevance to analyze simultaneously the microRNA and target mRNA fates to understand their role in promoting an adequate embryogenic response to external stimulus in the regenerating tissues. Here we describe a method to evaluate the expression patterns of miRNAs or other sRNAs and their target regulation in distinctive tissues observed during maize plant regeneration. Key features of the method include the classification of regenerating plant material with reproducibly distinctive morphological characteristics and a purification procedure that renders high-quality small and large RNA separation from the same sample for qRT-PCR analysis. |
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