Chapter title |
Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer
|
---|---|
Chapter number | 21 |
Book title |
Teratogenicity Testing
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7883-0_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7882-3, 978-1-4939-7883-0
|
Authors |
Simon T. Bond, Kevin A. McEwen, Prusothman Yoganantharajah, Yann Gibert |
Abstract |
The Agilent Seahorse Extracellular Flux Analyzer can be used to measure the oxygen consumption rate (OCR) and extra cellular acidification rate (ECAR), from which mitochondrial bioenergetics measurements can be determined including basal respiration, respiration due to ATP turnover, uncoupled respiration/proton leak, and maximum respiration. This novel method demonstrates how to use a Seahorse XF 24 Extracellular Flux Analyzer to measure the bioenergetic flux of zebrafish embryos in vivo during development. This provides a tool that enables characterization of metabolic parameters in a living organism, utilizing Agilent Islet Capture Microplates where respiration parameters can be compared between controls and genetically altered/pharmacologically treated embryos in real time. This method can be used to analyze and identify novel pharmaceuticals and genes that influence respiration, mitochondrial function, and metabolism. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 44 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 14% |
Student > Bachelor | 6 | 14% |
Student > Ph. D. Student | 5 | 11% |
Student > Doctoral Student | 3 | 7% |
Other | 2 | 5% |
Other | 5 | 11% |
Unknown | 17 | 39% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 13 | 30% |
Agricultural and Biological Sciences | 5 | 11% |
Medicine and Dentistry | 3 | 7% |
Environmental Science | 2 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 4 | 9% |
Unknown | 16 | 36% |