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ADP-ribosylation and NAD+ Utilizing Enzymes

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Cover of 'ADP-ribosylation and NAD+ Utilizing Enzymes'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Vitamin B3 in Health and Disease: Toward the Second Century of Discovery
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    Chapter 2 Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently Tagged Macrodomains
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    Chapter 3 In Vitro Techniques for ADP-Ribosylated Substrate Identification
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    Chapter 4 Assessment of Intracellular Auto-Modification Levels of ARTD10 Using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8
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    Chapter 5 Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR-Binding Analysis
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    Chapter 6 Assays for NAD+-Dependent Reactions and NAD+ Metabolites
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    Chapter 7 Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro
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    Chapter 8 Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity
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    Chapter 9 Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events
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    Chapter 10 Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases
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    Chapter 11 Monitoring Expression and Enzyme Activity of Ecto-ARTCs
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    Chapter 12 ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins
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    Chapter 13 Mono-ADP-Ribosylhydrolase Assays
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    Chapter 14 Hydrolysis of ADP-Ribosylation by Macrodomains
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    Chapter 15 HPLC-Based Enzyme Assays for Sirtuins
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    Chapter 16 Small-Molecule Screening Assay for Mono-ADP-Ribosyltransferases
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    Chapter 17 A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors
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    Chapter 18 Nonlocalized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides
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    Chapter 19 Quantitative Determination of MAR Hydrolase Residue Specificity In Vitro by Tandem Mass Spectrometry
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    Chapter 20 Detection of ADP-Ribosylating Bacterial Toxins
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    Chapter 21 Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation
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    Chapter 22 Monitoring the Sensitivity of T Cell Populations Towards NAD+ Released During Cell Preparation
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    Chapter 23 Identifying Target RNAs of PARPs
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    Chapter 24 ADPr-Peptide Synthesis
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    Chapter 25 Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP
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    Chapter 26 Methods for Using a Genetically Encoded Fluorescent Biosensor to Monitor Nuclear NAD +
Attention for Chapter 10: Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases
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Chapter title
Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases
Chapter number 10
Book title
ADP-ribosylation and NAD+ Utilizing Enzymes
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8588-3_10
Pubmed ID
Book ISBNs
978-1-4939-8587-6, 978-1-4939-8588-3
Authors

Linda A. Stevens, Joel Moss, Stevens, Linda A., Moss, Joel

Abstract

Methods are described for determination of arginine-specific mono-ADP-ribosyltransferase activity of purified proteins and intact cells by monitoring the transfer of ADP-ribose from NAD+ to a model substrate, e.g., arginine, agmatine, and peptide (human neutrophil peptide-1 [HNP1]), and for the nonenzymatic hydrolysis of ADP-ribose-arginine to ornithine, a noncoded amino acid. In addition, preparation of purified ADP-ribosylarginine is included as a control substrate for ADP-ribosylation reactions.

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Mendeley readers

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The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 50%
Student > Master 1 50%
Readers by discipline Count As %
Chemistry 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 March 2022.
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#22,757,832
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#10,997
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#296,374
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Outputs of similar age from Methods in molecular biology
#185
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