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ADP-ribosylation and NAD+ Utilizing Enzymes

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Cover of 'ADP-ribosylation and NAD+ Utilizing Enzymes'

Table of Contents

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    Book Overview
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    Chapter 1 Vitamin B3 in Health and Disease: Toward the Second Century of Discovery
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    Chapter 2 Monitoring Poly(ADP-Ribosyl)ation in Response to DNA Damage in Live Cells Using Fluorescently Tagged Macrodomains
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    Chapter 3 In Vitro Techniques for ADP-Ribosylated Substrate Identification
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    Chapter 4 Assessment of Intracellular Auto-Modification Levels of ARTD10 Using Mono-ADP-Ribose-Specific Macrodomains 2 and 3 of Murine Artd8
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    Chapter 5 Biochemical and Biophysical Assays of PAR-WWE Domain Interactions and Production of iso-ADPr for PAR-Binding Analysis
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    Chapter 6 Assays for NAD+-Dependent Reactions and NAD+ Metabolites
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    Chapter 7 Generating Protein-Linked and Protein-Free Mono-, Oligo-, and Poly(ADP-Ribose) In Vitro
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    Chapter 8 Methods to Study TCDD-Inducible Poly-ADP-Ribose Polymerase (TIPARP) Mono-ADP-Ribosyltransferase Activity
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    Chapter 9 Dictyostelium as a Model to Assess Site-Specific ADP-Ribosylation Events
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    Chapter 10 Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases
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    Chapter 11 Monitoring Expression and Enzyme Activity of Ecto-ARTCs
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    Chapter 12 ADP-Ribosyl-Acceptor Hydrolase Activities Catalyzed by the ARH Family of Proteins
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    Chapter 13 Mono-ADP-Ribosylhydrolase Assays
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    Chapter 14 Hydrolysis of ADP-Ribosylation by Macrodomains
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    Chapter 15 HPLC-Based Enzyme Assays for Sirtuins
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    Chapter 16 Small-Molecule Screening Assay for Mono-ADP-Ribosyltransferases
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    Chapter 17 A Simple, Sensitive, and Generalizable Plate Assay for Screening PARP Inhibitors
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    Chapter 18 Nonlocalized Searching of HCD Data for Fast and Sensitive Identification of ADP-Ribosylated Peptides
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    Chapter 19 Quantitative Determination of MAR Hydrolase Residue Specificity In Vitro by Tandem Mass Spectrometry
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    Chapter 20 Detection of ADP-Ribosylating Bacterial Toxins
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    Chapter 21 Preparation of Recombinant Alphaviruses for Functional Studies of ADP-Ribosylation
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    Chapter 22 Monitoring the Sensitivity of T Cell Populations Towards NAD+ Released During Cell Preparation
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    Chapter 23 Identifying Target RNAs of PARPs
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    Chapter 24 ADPr-Peptide Synthesis
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    Chapter 25 Identifying Genomic Sites of ADP-Ribosylation Mediated by Specific Nuclear PARP Enzymes Using Click-ChIP
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    Chapter 26 Methods for Using a Genetically Encoded Fluorescent Biosensor to Monitor Nuclear NAD +
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