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Toll-Like Receptors

Overview of attention for book
Toll-Like Receptors
Humana Press, New York, NY

Table of Contents

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    Book Overview
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    Chapter 1 Toll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action
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    Chapter 2 Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures.
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    Chapter 3 Toll-Like Receptor Interactions Measured by Microscopic and Flow Cytometric FRET
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    Chapter 4 Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors
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    Chapter 5 Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing.
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    Chapter 6 Methods for Delivering DNA to Intracellular Receptors
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    Chapter 7 Detection of Interaction Between Toll-Like Receptors and Other Transmembrane Proteins by Co-immunoprecipitation Assay
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    Chapter 8 Flow Cytometry-Based Bead-Binding Assay for Measuring Receptor Ligand Specificity
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    Chapter 9 Measuring Monomer-to-Filament Transition of MAVS as an In Vitro Activity Assay for RIG-I-Like Receptors
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    Chapter 10 Co-transcriptomic Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression in Host and Bacterial Pathogen
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    Chapter 11 Simple Methods to Investigate MicroRNA Induction in Response to Toll-Like Receptors.
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    Chapter 12 Determining the Function of Long Noncoding RNA in Innate Immunity.
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    Chapter 13 Analysis of Post-transcriptional Gene Regulation of Nod-Like Receptors via the 3'UTR.
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    Chapter 14 TLR Function in Murine CD4+ T Lymphocytes and Their Role in Inflammation
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    Chapter 15 Analysis by Flow Cytometry of B-Cell Activation and Antibody Responses Induced by Toll-Like Receptors.
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    Chapter 16 Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells.
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    Chapter 17 Analysis of TLR-Induced Metabolic Changes in Dendritic Cells Using the Seahorse XF(e)96 Extracellular Flux Analyzer.
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    Chapter 18 Toll-Like Receptor Signalling and the Control of Intestinal Barrier Function
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    Chapter 19 Understanding the Role of Cellular Molecular Clocks in Controlling the Innate Immune Response.
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    Chapter 20 Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis
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    Chapter 21 Allergens and Activation of the Toll-Like Receptor Response.
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    Chapter 22 Investigating the Role of Toll-Like Receptors in Models of Arthritis
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    Chapter 23 Delineating the Role of Toll-Like Receptors in the Neuro-inflammation Model EAE.
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    Chapter 24 The Use of MiRNA Antagonists in the Alleviation of Inflammatory Disorders.
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    Chapter 25 Investigating the Role of Toll-Like Receptors in Mouse Models of Gastric Cancer
Attention for Chapter 4: Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors
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Chapter title
Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors
Chapter number 4
Book title
Toll-Like Receptors
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3335-8_4
Pubmed ID
26803622
Book ISBNs
978-1-4939-3333-4, 978-1-4939-3335-8
Authors

Harald Husebye, Sarah L. Doyle

Abstract

Toll-like receptors (TLR) survey the extracellular space, cytoplasm, and endosomal compartments for signs of infection or tissue injury. Over the past decade, it has become evident that TLR activation and signal transduction can be regulated by subcellular compartmentalization of both the receptors and their downstream signaling components. Immunofluorescence and/or overexpression of fluorescently "tagged"' proteins teamed with confocal microscopy presents a powerful technique for studying the spatial organization of TLRs, their signaling mediators, and the dynamic processes they activate. This chapter details the common methods for determining the subcellular location of TLRs in both live and fixed cells.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 29%
Student > Master 2 29%
Student > Bachelor 1 14%
Student > Postgraduate 1 14%
Lecturer 1 14%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 57%
Unspecified 1 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 14%
Immunology and Microbiology 1 14%

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