| Chapter title |
Genetic Interaction Scoring Procedure for Bacterial Species
|
|---|---|
| Chapter number | 10 |
| Book title |
Prokaryotic Systems Biology
|
| Published in |
Advances in experimental medicine and biology, January 2015
|
| DOI | 10.1007/978-3-319-23603-2_10 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-923602-5, 978-3-31-923603-2
|
| Authors |
Omar Wagih, Leopold Parts |
| Abstract |
A genetic interaction occurs when the phenotype of an organism carrying two mutant genes differs from what should have been observed given their independent influence. Such unexpected outcome indicates a mechanistic connection between the perturbed genes, providing a key source of functional information about the cell. Large-scale screening for genetic interactions involves measuring phenotypes of single and double mutants, which for microorganisms is usually done by automated analysis of images of ordered colonies. Obtaining accurate colony sizes, and using them to identify genetic interactions from such screens remains a challenging and time-consuming task. Here, we outline steps to compute genetic interaction scores in E. coli by measuring colony sizes from plate images, performing normalisation, and quantifying the strength of the effect. |
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