Chapter title |
High-Resolution Melt Curve Analysis in Cancer Mutation Screen
|
---|---|
Chapter number | 7 |
Book title |
Clinical Applications of PCR
|
Published in |
Methods in molecular biology, February 2016
|
DOI | 10.1007/978-1-4939-3360-0_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3358-7, 978-1-4939-3360-0
|
Authors |
Mehrotra, Meenakshi, Patel, Keyur P., Meenakshi Mehrotra, Keyur P. Patel M.D., Ph.D., Keyur P. Patel |
Editors |
Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel |
Abstract |
High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 17% |
Student > Ph. D. Student | 2 | 17% |
Student > Master | 1 | 8% |
Researcher | 1 | 8% |
Student > Postgraduate | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |
Readers by discipline | Count | As % |
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Earth and Planetary Sciences | 1 | 8% |
Medicine and Dentistry | 1 | 8% |
Unknown | 6 | 50% |