| Chapter title |
Quantitative Real-Time PCR: Recent Advances
|
|---|---|
| Chapter number | 15 |
| Book title |
Clinical Applications of PCR
|
| Published in |
Methods in molecular biology, February 2016
|
| DOI | 10.1007/978-1-4939-3360-0_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3358-7, 978-1-4939-3360-0
|
| Authors |
Singh, Charanjeet, Roy-Chowdhuri, Sinchita, Charanjeet Singh, Sinchita Roy-Chowdhuri |
| Editors |
Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel |
| Abstract |
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or second sequence is typically adequate. The quantification process employs melting curve analysis and/or fluorescent detection systems and can provide amplification and genotyping in a relatively short time. Here we describe the properties and uses of various fluorescent detection systems used for quantification. |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Hungary | 1 | <1% |
| Sweden | 1 | <1% |
| Sri Lanka | 1 | <1% |
| Unknown | 230 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 41 | 18% |
| Student > Master | 31 | 13% |
| Student > Ph. D. Student | 17 | 7% |
| Researcher | 15 | 6% |
| Student > Doctoral Student | 11 | 5% |
| Other | 25 | 11% |
| Unknown | 93 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 57 | 24% |
| Agricultural and Biological Sciences | 21 | 9% |
| Medicine and Dentistry | 19 | 8% |
| Immunology and Microbiology | 8 | 3% |
| Veterinary Science and Veterinary Medicine | 6 | 3% |
| Other | 23 | 10% |
| Unknown | 99 | 42% |