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Clinical Applications of PCR

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Cover of 'Clinical Applications of PCR'

Table of Contents

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    Book Overview
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    Chapter 1 A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care.
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    Chapter 2 COLD-PCR: Applications and Advantages
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    Chapter 3 PCR-Based Detection of DNA Copy Number Variation.
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    Chapter 4 Emulsion PCR: Techniques and Applications
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    Chapter 5 Digital PCR: Principles and Applications
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    Chapter 6 Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics
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    Chapter 7 High-Resolution Melt Curve Analysis in Cancer Mutation Screen
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    Chapter 8 Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations
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    Chapter 9 Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis
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    Chapter 10 Microfluidics-Based PCR for Fusion Transcript Detection.
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    Chapter 11 Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach
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    Chapter 12 Detection of Trypanosoma cruzi by Polymerase Chain Reaction
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    Chapter 13 PCR Techniques in Next-Generation Sequencing.
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    Chapter 14 Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics
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    Chapter 15 Quantitative Real-Time PCR: Recent Advances
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    Chapter 16 PCR Techniques in Characterizing DNA Methylation.
Attention for Chapter 14: Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics
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Chapter title
Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics
Chapter number 14
Book title
Clinical Applications of PCR
Published in
Methods in molecular biology, February 2016
DOI 10.1007/978-1-4939-3360-0_14
Pubmed ID
Book ISBNs
978-1-4939-3358-7, 978-1-4939-3360-0
Authors

Ok, Chi Young, Singh, Rajesh R., Salim, Alaa A., Chi Young Ok, Rajesh R. Singh, Alaa A. Salim

Editors

Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel

Abstract

Tissues are heterogeneous in their components. If cells of interest are a minor population of collected tissue, it would be difficult to obtain genetic or genomic information of the interested cell population with conventional genomic DNA extraction from the collected tissue. Single-cell DNA analysis is important in the analysis of genetics of cell clonality, genetic anticipation, and single-cell DNA polymorphisms. Single-cell PCR using Single Cell Ampligrid/GeXP platform is described in this chapter.

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Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 25%
Other 1 25%
Student > Master 1 25%
Unknown 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 25%
Engineering 1 25%
Unknown 2 50%