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Clinical Applications of PCR

Overview of attention for book
Cover of 'Clinical Applications of PCR'

Table of Contents

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    Book Overview
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    Chapter 1 A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care.
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    Chapter 2 COLD-PCR: Applications and Advantages
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    Chapter 3 PCR-Based Detection of DNA Copy Number Variation.
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    Chapter 4 Emulsion PCR: Techniques and Applications
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    Chapter 5 Digital PCR: Principles and Applications
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    Chapter 6 Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics
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    Chapter 7 High-Resolution Melt Curve Analysis in Cancer Mutation Screen
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    Chapter 8 Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations
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    Chapter 9 Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis
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    Chapter 10 Microfluidics-Based PCR for Fusion Transcript Detection.
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    Chapter 11 Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach
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    Chapter 12 Detection of Trypanosoma cruzi by Polymerase Chain Reaction
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    Chapter 13 PCR Techniques in Next-Generation Sequencing.
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    Chapter 14 Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics
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    Chapter 15 Quantitative Real-Time PCR: Recent Advances
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    Chapter 16 PCR Techniques in Characterizing DNA Methylation.
Attention for Chapter 11: Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach
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Chapter title
Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach
Chapter number 11
Book title
Clinical Applications of PCR
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3360-0_11
Pubmed ID
26843051
Book ISBNs
978-1-4939-3358-7, 978-1-4939-3360-0
Authors

González-Marcano, Eglys, Kato, Hirotomo, Concepción, Juan Luis, Márquez, María Elizabeth, Mondolfi, Alberto Paniz, Eglys González-Marcano, Hirotomo Kato, Juan Luis Concepción, María Elizabeth Márquez, Alberto Paniz Mondolfi

Editors

Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel

Abstract

Leishmaniasis is an infectious disease caused by protozoan parasites of the genus Leishmania which are transmitted to humans through bites of infected sand flies. The variable clinical manifestations and the evolution of the disease are determined by the infecting species. Recognition at a species level is of utmost importance since this greatly impacts therapy decision making as well as predicts outcome for the disease. This chapter describes the application of polymerase chain reaction (PCR) in the detection of Leishmania parasites across the disease spectrum, including protocols for sample collection and transportation, genomic material extraction, and target amplification methods with special emphasis on PCR amplification of the cytochrome b gene for Leishmania spp. species identification.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Professor 4 40%
Student > Bachelor 2 20%
Student > Doctoral Student 1 10%
Student > Master 1 10%
Researcher 1 10%
Other 0 0%
Unknown 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 30%
Biochemistry, Genetics and Molecular Biology 2 20%
Nursing and Health Professions 1 10%
Veterinary Science and Veterinary Medicine 1 10%
Immunology and Microbiology 1 10%
Other 1 10%
Unknown 1 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2016.
All research outputs
#18,441,836
of 22,849,304 outputs
Outputs from Methods in molecular biology
#7,922
of 13,127 outputs
Outputs of similar age
#284,428
of 393,581 outputs
Outputs of similar age from Methods in molecular biology
#846
of 1,470 outputs
Altmetric has tracked 22,849,304 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.

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