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Clinical Applications of PCR

Overview of attention for book
Cover of 'Clinical Applications of PCR'

Table of Contents

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    Book Overview
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    Chapter 1 A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care.
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    Chapter 2 COLD-PCR: Applications and Advantages
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    Chapter 3 PCR-Based Detection of DNA Copy Number Variation.
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    Chapter 4 Emulsion PCR: Techniques and Applications
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    Chapter 5 Digital PCR: Principles and Applications
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    Chapter 6 Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics
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    Chapter 7 High-Resolution Melt Curve Analysis in Cancer Mutation Screen
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    Chapter 8 Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations
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    Chapter 9 Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis
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    Chapter 10 Microfluidics-Based PCR for Fusion Transcript Detection.
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    Chapter 11 Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach
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    Chapter 12 Detection of Trypanosoma cruzi by Polymerase Chain Reaction
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    Chapter 13 PCR Techniques in Next-Generation Sequencing.
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    Chapter 14 Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics
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    Chapter 15 Quantitative Real-Time PCR: Recent Advances
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    Chapter 16 PCR Techniques in Characterizing DNA Methylation.
Attention for Chapter 10: Microfluidics-Based PCR for Fusion Transcript Detection.
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Chapter title
Microfluidics-Based PCR for Fusion Transcript Detection.
Chapter number 10
Book title
Clinical Applications of PCR
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3360-0_10
Pubmed ID
26843050
Book ISBNs
978-1-4939-3358-7, 978-1-4939-3360-0
Authors

Chen, Hui, Hui Chen M.D., Ph.D., Hui Chen

Editors

Rajyalakshmi Luthra, Rajesh R. Singh, Keyur P. Patel

Abstract

The microfluidic technology allows the production of network of submillimeter-size fluidic channels and reservoirs in a variety of material systems. The microfluidic-based polymerase chain reaction (PCR) allows automated multiplexing of multiple samples and multiple assays simultaneously within a network of microfluidic channels and chambers that are co-ordinated in controlled fashion by the valves. The individual PCR reaction is performed in nanoliter volume, which allows testing on samples with limited DNA and RNA. The microfluidics devices are used in various types of PCR such as digital PCR and single molecular emulsion PCR for genotyping, gene expression, and miRNA expression. In this chapter, the use of a microfluidics-based PCR for simultaneous screening of 14 known fusion transcripts in patients with leukemia is described.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 40%
Researcher 1 20%
Student > Master 1 20%
Unknown 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Agricultural and Biological Sciences 1 20%
Unknown 2 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2016.
All research outputs
#20,305,223
of 22,844,985 outputs
Outputs from Methods in molecular biology
#9,917
of 13,127 outputs
Outputs of similar age
#330,614
of 393,571 outputs
Outputs of similar age from Methods in molecular biology
#1,053
of 1,470 outputs
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So far Altmetric has tracked 13,127 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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