Chapter title |
TUBEs-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome.
|
---|---|
Chapter number | 9 |
Book title |
Proteostasis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3756-1_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3754-7, 978-1-4939-3756-1
|
Authors |
Mikel Azkargorta, Iraide Escobes, Felix Elortza, Rune Matthiesen, Manuel S. Rodríguez |
Editors |
Rune Matthiesen |
Abstract |
Mass spectrometry (MS) has become the method of choice for the large-scale analysis of protein ubiquitylation. There exist a number of proposed methods for mapping ubiquitin sites, each with different pros and cons. We present here a protocol for the MS analysis of the ubiquitin-proteome captured by TUBEs and subsequent data analysis. Using dedicated software and algorithms, specific information on the presence of ubiquitylated peptides can be obtained from the MS search results. In addition, a quantitative and functional analysis of the ubiquitylated proteins and their interacting partners helps to unravel the biological and molecular processes they are involved in. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 25% |
United Kingdom | 1 | 25% |
Australia | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 35% |
Researcher | 6 | 26% |
Student > Master | 2 | 9% |
Student > Doctoral Student | 1 | 4% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 1 | 4% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 14 | 61% |
Agricultural and Biological Sciences | 2 | 9% |
Medicine and Dentistry | 1 | 4% |
Neuroscience | 1 | 4% |
Chemistry | 1 | 4% |
Other | 0 | 0% |
Unknown | 4 | 17% |