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Chapter title |
Method for the Purification of Endogenous Unanchored Polyubiquitin Chains.
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Chapter number | 11 |
Book title |
Proteostasis
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-3756-1_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3754-7, 978-1-4939-3756-1
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Authors |
Daniel Scott, Jo Strachan, Varun Gopala Krishna, Barry Shaw, David J. Tooth, Mark S. Searle, Neil J. Oldham, Rob Layfield |
Editors |
Rune Matthiesen |
Abstract |
Unanchored polyubiquitin chains are endogenous non-substrate linked ubiquitin polymers which have emerging roles in the control of cellular physiology. We describe an affinity purification method based on an isolated ubiquitin-binding domain, the ZnF_UBP domain of the deubiquitinating enzyme USP5, which permits the selective purification of mixtures of endogenous unanchored polyubiquitin chains that are amenable to downstream molecular analyses. Further, we present methods for detection of unanchored polyubiquitin chains in purified fractions. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 40% |
Researcher | 2 | 40% |
Student > Postgraduate | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 80% |
Chemistry | 1 | 20% |