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Proteostasis

Overview of attention for book
Cover of 'Proteostasis'

Table of Contents

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    Book Overview
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    Chapter 1 UPS Activation in the Battle Against Aging and Aggregation-Related Diseases: An Extended Review.
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    Chapter 2 Proteostasis
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    Chapter 3 Proteostasis
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    Chapter 4 Immunodepletion and Immunopurification as Approaches for CSN Research.
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    Chapter 5 Proteostasis
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    Chapter 6 Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein.
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    Chapter 7 In Vitro Ubiquitination: Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays.
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    Chapter 8 Isolation of the Ubiquitin-Proteome from Tumor Cell Lines and Primary Cells Using TUBEs.
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    Chapter 9 TUBEs-Mass Spectrometry for Identification and Analysis of the Ubiquitin-Proteome.
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    Chapter 10 Isolation of Ubiquitinated Proteins to High Purity from In Vivo Samples.
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    Chapter 11 Method for the Purification of Endogenous Unanchored Polyubiquitin Chains.
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    Chapter 12 Proteostasis
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    Chapter 13 Proteostasis
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    Chapter 14 Monitoring Ubiquitin-Coated Bacteria via Confocal Microscopy
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    Chapter 15 Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo.
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    Chapter 16 Detection and Analysis of SUMOylation Substrates In Vitro and In Vivo.
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    Chapter 17 Detection of Protein-Protein Interactions and Posttranslational Modifications Using the Proximity Ligation Assay: Application to the Study of the SUMO Pathway.
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    Chapter 18 Dissecting SUMO Dynamics by Mass Spectrometry.
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    Chapter 19 Proteostasis
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    Chapter 20 Proteostasis
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    Chapter 21 Proteostasis
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    Chapter 22 Analysis of Protein Oligomerization by Electrophoresis.
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    Chapter 23 Blot-MS of Carbonylated Proteins: A Tool to Identify Oxidized Proteins.
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    Chapter 24 Proteostasis
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    Chapter 25 A Simple Protocol for High Efficiency Protein Isolation After RNA Isolation from Mouse Thyroid and Other Very Small Tissue Samples.
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    Chapter 26 Monitoring Target Engagement of Deubiquitylating Enzymes Using Activity Probes: Past, Present, and Future.
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    Chapter 27 Proteostasis
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    Chapter 28 Proteostasis
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    Chapter 29 Proteostasis
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    Chapter 30 Using AlphaScreen(®) to Identify Small-Molecule Inhibitors Targeting a Conserved Host-Pathogen Interaction.
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    Chapter 31 Proteostasis
Attention for Chapter 18: Dissecting SUMO Dynamics by Mass Spectrometry.
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Chapter title
Dissecting SUMO Dynamics by Mass Spectrometry.
Chapter number 18
Book title
Proteostasis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3756-1_18
Pubmed ID
Book ISBNs
978-1-4939-3754-7, 978-1-4939-3756-1
Authors

Krzysztof Drabikowski Ph.D., Michał Dadlez, Krzysztof Drabikowski

Editors

Rune Matthiesen

Abstract

Protein modification by SUMO proteins is one of the key posttranslational modifications in eukaryotes. Here, we describe a workflow to analyze SUMO dynamics in response to different stimuli, purify SUMO conjugates, and analyze the changes in SUMOylation level in organisms, tissues, or cell culture. We present a protocol for lysis in denaturing conditions that is compatible with downstream IMAC and antibody affinity purification, followed by mass spectrometry and data analysis.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 20%
Student > Ph. D. Student 1 20%
Professor > Associate Professor 1 20%
Researcher 1 20%
Lecturer > Senior Lecturer 1 20%
Other 0 0%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 40%
Unspecified 1 20%
Chemistry 1 20%
Unknown 1 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 January 2018.
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#20,341,859
of 22,888,307 outputs
Outputs from Methods in molecular biology
#9,921
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Outputs of similar age
#330,770
of 393,716 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
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So far Altmetric has tracked 13,132 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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