Phage Display

Chapter 1 Construction of Human Immune and Naive scFv Libraries

Chapter 2 Construction of Naive and Immune Human Fab Phage-Display Library

Chapter 3 Construction of Synthetic Antibody Phage-Display Libraries

Chapter 4 Modular Construction of Large Non-Immune Human Antibody Phage-Display Libraries from Variable Heavy and Light Chain Gene Cassettes

Chapter 5 Construction of Macaque Immune-Libraries

Chapter 6 Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format

Chapter 7 Construction of Rabbit Immune Antibody Libraries

Chapter 8 Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries

Chapter 9 Construction of High-Quality Camel Immune Antibody Libraries

Chapter 10 Construction of Chicken Antibody Libraries

Chapter 11 Construction and Selection of Affilin® Phage Display Libraries

Chapter 12 Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies

Chapter 13 Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach

Chapter 14 Parallelized Antibody Selection in Microtiter Plates

Chapter 15 Mass Spectrometry Immuno Assay (MSIA™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning

Chapter 16 Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection

Chapter 17 Phage Display and Selections on Cells

Chapter 18 Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens

Chapter 19 Antibody Isolation From a Human Synthetic Combinatorial and Other Libraries of Single-Chain Antibodies

Chapter 20 Screening Phage-Display Antibody Libraries Using Protein Arrays

Chapter 21 Antibody Selection on FFPE Tissue Slides

Chapter 22 Antibody Affinity and Stability Maturation by Error-Prone PCR

Chapter 23 Upgrading Affinity Screening Experiments by Analysis of Next-Generation Sequencing Data

Chapter 24 Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Discovery

Chapter 25 High-Throughput IgG Reformatting and Expression

Chapter 26 Monitoring Phage Biopanning by Next-Generation Sequencing

Chapter 27 ORFeome Phage Display.

Chapter 28 Epitope Mapping by Phage Display

Chapter 29 Metasecretome Phage Display

Chapter 30 Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages