| Chapter title |
Detecting Expressed Genes Using CAGE.
|
|---|---|
| Chapter number | 7 |
| Book title |
Transcription Factor Regulatory Networks
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4939-0805-9_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-0804-2, 978-1-4939-0805-9
|
| Authors |
Mitsuyoshi Murata, Hiromi Nishiyori-Sueki, Miki Kojima-Ishiyama, Piero Carninci, Yoshihide Hayashizaki, Masayoshi Itoh, Murata, Mitsuyoshi, Nishiyori-Sueki, Hiromi, Kojima-Ishiyama, Miki, Carninci, Piero, Hayashizaki, Yoshihide, Itoh, Masayoshi |
| Abstract |
Cap analysis of gene expression (CAGE) provides accurate high-throughput measurement of RNA expression. By the large-scale analysis of 5' end of transcripts using CAGE method, it enables not only determination of the transcription start site but also prediction of promoter region. Here we provide a protocol for the construction of no-amplification non-tagging CAGE libraries for Illumina next-generation sequencers (nAnT-iCAGE). We have excluded the commonly used PCR amplification and cleavage of restriction enzyme to eliminate any potential biases. As a result, we achieved less biased simple preparation process. |
Mendeley readers
Geographical breakdown
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|---|---|---|
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Demographic breakdown
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|---|---|---|
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| Student > Ph. D. Student | 22 | 23% |
| Student > Bachelor | 8 | 9% |
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| Student > Postgraduate | 6 | 6% |
| Other | 12 | 13% |
| Unknown | 16 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Other | 10 | 11% |
| Unknown | 15 | 16% |