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Germline Stem Cells

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Cover of 'Germline Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of the C. elegans Germline Stem Cell Pool.
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    Chapter 2 Methods for Studying the Germline of the Human Parasite Schistosoma mansoni.
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    Chapter 3 Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells.
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    Chapter 4 Live Imaging of the Drosophila Testis Stem Cell Niche.
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    Chapter 5 RNA Isolation from Early Drosophila Larval Ovaries.
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    Chapter 6 Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy.
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    Chapter 7 Measurement of mRNA Poly(A) Tail Lengths in Drosophila Female Germ Cells and Germ-Line Stem Cells.
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    Chapter 8 Identification of Germ-Line Stem Cells in Zebrafish.
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    Chapter 9 Primordial Germ Cell Isolation from Xenopus laevis Embryos.
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    Chapter 10 Single-Cell Lineage Analysis of Oogenesis in Mice.
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    Chapter 11 Visualization and Lineage Tracing of Pax7(+) Spermatogonial Stem Cells in the Mouse.
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    Chapter 12 Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells.
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    Chapter 13 Mouse Fetal Germ Cell Isolation and Culture Techniques.
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    Chapter 14 Rattus norvegicus Spermatogenesis Colony-Forming Assays.
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    Chapter 15 Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies.
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    Chapter 16 Efficient Induction and Isolation of Human Primordial Germ Cell-Like Cells from Competent Human Pluripotent Stem Cells.
Attention for Chapter 8: Identification of Germ-Line Stem Cells in Zebrafish.
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Chapter title
Identification of Germ-Line Stem Cells in Zebrafish.
Chapter number 8
Book title
Germline Stem Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-4017-2_8
Pubmed ID
27734351
Book ISBNs
978-1-4939-4015-8, 978-1-4939-4017-2
Authors

Bruce W. Draper

Editors

Michael Buszczak

Abstract

Both male and female zebrafish have a population of germ-line stem cells that produce gametes throughout the life of the fish. These cells localize to specific regions in the gonads and can be identified because they uniquely express the nanos2 gene, which encodes a conserved regulator of translation. A method is presented here for identifying germ-line stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect nanos2 mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.

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Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 31%
Student > Bachelor 2 6%
Professor > Associate Professor 2 6%
Lecturer 1 3%
Professor 1 3%
Other 3 9%
Unknown 13 41%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 28%
Agricultural and Biological Sciences 6 19%
Medicine and Dentistry 2 6%
Veterinary Science and Veterinary Medicine 1 3%
Unknown 14 44%

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