| Chapter title |
Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells.
|
|---|---|
| Chapter number | 12 |
| Book title |
Germline Stem Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-4017-2_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4015-8, 978-1-4939-4017-2
|
| Authors |
Aileen R. Helsel, Jon M. Oatley |
| Editors |
Michael Buszczak |
| Abstract |
In mammals, the activities of spermatogonial stem cells (SSCs) provide the foundation for continual spermatogenesis throughout a male's reproductive lifetime. At present, the defining characteristics of SSCs and mechanisms controlling their fate decisions are not well understood. Transplantation is a definitive functional measure of stem cell capacity for male germ cells that can be used as an assay to provide an unequivocal quantification of the SSC content in an experimental cell population. Here, we discuss the procedure for mice and provide protocols for preparing donor germ cell suspensions from testes directly or primary cultures of spermatogonia for transplantation, enriching for SSCs, preparing recipient males, microinjection into recipient testes, and considerations for experimental design. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 39% |
| Other | 2 | 11% |
| Student > Doctoral Student | 2 | 11% |
| Researcher | 2 | 11% |
| Professor | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 3 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 22% |
| Agricultural and Biological Sciences | 4 | 22% |
| Neuroscience | 3 | 17% |
| Medicine and Dentistry | 1 | 6% |
| Social Sciences | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 5 | 28% |