| Chapter title |
Mouse Fetal Germ Cell Isolation and Culture Techniques.
|
|---|---|
| Chapter number | 13 |
| Book title |
Germline Stem Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-4017-2_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4015-8, 978-1-4939-4017-2
|
| Authors |
Cassy M. Spiller, Guillaume Burnet, Josephine Bowles |
| Editors |
Michael Buszczak |
| Abstract |
The fetal gonad contains a great variety of differentiating cell populations, of which germ cells make up a small percentage. In order to study germ cell-specific gene and protein expression, as well as determine direct effects of signaling molecules, it is necessary to prepare enriched populations of germ cells and maintain them in culture for several hours to multiple days. The protocols in this chapter are designed to provide a guide for the isolation or enrichment of mouse primordial germ cells (from 9.5 days postcoitum (dpc) to 18.5 dpc) by flow cytometry (Subheading 3.1) or magnetic sorting (Subheading 3.2), followed by primary germ cell culture (Subheading 3.3). |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 10% |
| Unknown | 9 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 30% |
| Student > Ph. D. Student | 2 | 20% |
| Student > Master | 2 | 20% |
| Lecturer > Senior Lecturer | 1 | 10% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 60% |
| Agricultural and Biological Sciences | 2 | 20% |
| Unknown | 2 | 20% |