Chapter title |
Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment.
|
---|---|
Chapter number | 4 |
Book title |
Metagenomics
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6691-2_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6689-9, 978-1-4939-6691-2
|
Authors |
Mikkel A. Glaring, Jan K. Vester, Peter Stougaard |
Editors |
Wolfgang R. Streit, Rolf Daniel |
Abstract |
Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns. |
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